Record assay conditions alongside the measurements used to construct each response curve.
01
Record assay conditions
Record the cell model, assay, treatment, concentration units, exposure time, and replicate structure.
02
Review measurements
Keep individual observations available alongside summaries. Document how background correction and normalization were performed.
03
Review response curves
Review the observed range and model assumptions before reporting concentration response parameters.
Methods & documentation
Assay methods and response definitions.
About this example
Points show simulated replicate values; lines show the simulation models. The example contains seven concentrations at each of three exposure times. Download the CSV to inspect individual values.
Measurement and interpretation
The assay, controls, normalization, and model definition belong with the reported result. The response label should reflect what the assay measures. A single response curve does not establish a mechanism of cell death.